Ontology:
https://www.proquest.com/dissertations-theses/elucidating-genetic-control-qualitative-traits/docview/2726488649/se-2
Comment:
A second sex determination assay was developed as it was noted that several female individuals had null results, making it not truly codominant and not well suited for distinguishing YY supermales. To address this, CSP-2 was developed through identifying genes expressed only in males from a male-derived transcriptome (Braich et al 2019), aligning them to a male contig-level genome (Jamaican Lion Father, unpublished), and aligning this contig (JAATIQ010000695.1) to the X chromosome (Grassa et al., 2021). SNPs were identified in Geneious Prime and validated to give identical results as CSP-1 on 500 plants, but to be strictly codominant and lacking nulls.